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Difference between revisions of "Field-Flow fractionation (FFF)"
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Field-Flow fractionation (FFF) (view source)
Revision as of 13:05, 2 September 2021
, 13:05, 2 September 2021→Process and technologies
Lars Krause (talk | contribs) |
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==Feedstock== | ==Feedstock== | ||
Suitable feedstocks are heterogeneous mixtures of different substances in form of dilute suspensions (solids in liquid). Depending on the applied process and technology solids can be usually separated between the nm-µm range. | Suitable feedstocks are heterogeneous mixtures of different substances in form of dilute suspensions (solids in liquid). Depending on the applied process and technology solids can be usually separated between the nm-µm range. The FFF is usually applied to separate nanoparticles, polymers, and proteins for analytical and preparative purposes. | ||
==Process and technologies== | ==Process and technologies== | ||
Different variants of the FFF are available including tha Asymmetric flow FFF, centrifugal FFF, electrical FFF, split flow thin-cell fractionation (SPLITT), and thermal FFF. | |||
===Asymmetric flow FFF (AF4)=== | ===Asymmetric flow FFF (AF4)=== | ||
[[File:AFFFF channel.svg|thumb|Illustration of a separation channel for asymmetric flow field-flow-fractionation.]] | |||
The asymmetric flow FFF (AF4) is realised in a separation channel where an separation force ist generated in form an asymmetric crossflow through a semipermeable membrane. The injected particles can be eluted in dependence of their physicochemical properties through a parabolic flow-profile in combination with the reduction of the crossflow. | |||
=== Centrifugal FFF === | === Centrifugal FFF === | ||