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Difference between revisions of "Field-Flow fractionation (FFF)"
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Field-Flow fractionation (FFF) (view source)
Revision as of 14:33, 2 September 2021
, 14:33, 2 September 2021→Asymmetric flow FFF (AF4)
Lars Krause (talk | contribs) |
Lars Krause (talk | contribs) |
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[[File:AFFFF channel.svg|thumb|Illustration of a separation channel for asymmetric flow field-flow-fractionation.]] | [[File:AFFFF channel.svg|thumb|Illustration of a separation channel for asymmetric flow field-flow-fractionation.]] | ||
[[File:FFF Separation Mechanism.webm|thumb|Animation of the AF4 separation driven by particle diffusion in a parabolic flow profile. Particles colored in red are the smaller and particles colored in blue are the larger ones. The force applied on the top is the crossflow (indicated by the arrows on the bottom). The elution flow in longitudinal direction is shown with the flow arrows indicating the velocity profile.]] | [[File:FFF Separation Mechanism.webm|thumb|Animation of the AF4 separation driven by particle diffusion in a parabolic flow profile. Particles colored in red are the smaller and particles colored in blue are the larger ones. The force applied on the top is the crossflow (indicated by the arrows on the bottom). The elution flow in longitudinal direction is shown with the flow arrows indicating the velocity profile.]] | ||
The asymmetric flow FFF (AF4) is realised in a separation channel where an separation force ist generated in form of an asymmetric crossflow through a semipermeable membrane. Through the cross-flow larger particles accumulate at the channel bottom while smaller particles accumulate more at the upper part induced by diffusion. The injected particles can be eluted through a parabolic flow-profile in combination with the reduction of the crossflow. | The asymmetric flow FFF (AF4) is realised in a separation channel where an separation force ist generated in form of an asymmetric crossflow through a semipermeable membrane and frit. Through the cross-flow larger particles accumulate at the channel bottom while smaller particles accumulate more at the upper part induced by diffusion. The injected particles can be eluted through a parabolic flow-profile in combination with the reduction of the crossflow. | ||
=== Centrifugal FFF === | === Centrifugal FFF === | ||