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For the flocculation of micro algae in wastewater, biopolymer flocculants can be used, as described in ''Microalgae-Based Biofuels and Bioproducts'', 2017: "Polymer flocculants are polymers with charged functional groups. Polymer flocculants can induce flocculation by neutralizing the surface charge of particles or by forming bridges between individual particles. The functional groups should ideally be positively charged to allow for interactions with the negatively charged microalgal cells. Polymers are generally very effective at low dosages. In wastewater treatment, polyacrylamide-based flocculants are commonly used. Because they can contain potentially toxic acrylamide residues, flocculants based on natural biopolymers are preferred over synthetic polymers. An effective biopolymer flocculant for harvesting microalgae is chitosan, which is prepared by deacetylation of chitin. However, the cost of chitosan is relatively high due to its use in medical applications. Cheaper alternatives are cationic starch or tanfloc, which are, respectively, starch and tannins functionalized with quaternary ammonium groups."<ref>{{Cite book|author=K. Muylaert, L. Bastiaens, D. Vandamme, L. Gouveia|year=2017|section_title=5 – Harvesting of microalgae: Overview of process options and their strengths and drawbacks – 5.3.5 Biopolymer flocculants|editor=Cristina Gonzalez-Fernandez, Raúl Muñoz|book_title=Microalgae-based biofuels and bioproducts : from feedstock cultivation to end-products|publisher=Woodhead Publishing|place=Kindlington, United Kingdom|ISBN=9780081010235}}</ref> | For the flocculation of micro algae in wastewater, biopolymer flocculants can be used, as described in ''Microalgae-Based Biofuels and Bioproducts'', 2017: "Polymer flocculants are polymers with charged functional groups. Polymer flocculants can induce flocculation by neutralizing the surface charge of particles or by forming bridges between individual particles. The functional groups should ideally be positively charged to allow for interactions with the negatively charged microalgal cells. Polymers are generally very effective at low dosages. In wastewater treatment, polyacrylamide-based flocculants are commonly used. Because they can contain potentially toxic acrylamide residues, flocculants based on natural biopolymers are preferred over synthetic polymers. An effective biopolymer flocculant for harvesting microalgae is chitosan, which is prepared by deacetylation of chitin. However, the cost of chitosan is relatively high due to its use in medical applications. Cheaper alternatives are cationic starch or tanfloc, which are, respectively, starch and tannins functionalized with quaternary ammonium groups."<ref>{{Cite book|author=K. Muylaert, L. Bastiaens, D. Vandamme, L. Gouveia|year=2017|section_title=5 – Harvesting of microalgae: Overview of process options and their strengths and drawbacks – 5.3.5 Biopolymer flocculants|editor=Cristina Gonzalez-Fernandez, Raúl Muñoz|book_title=Microalgae-based biofuels and bioproducts : from feedstock cultivation to end-products|publisher=Woodhead Publishing|place=Kindlington, United Kingdom|ISBN=9780081010235}}</ref> | ||
Exemplary applications | === Exemplary applications === | ||
Flocculation is used in biotechnology applications in conjunction with microfiltration to improve the efficiency of biological feeds. The addition of synthetic flocculants to the bioreactor can increase the average particle size making microfiltration more efficient. When flocculants are not added, cakes can form and accumulate causing low cell viability. Positively charged flocculants work better than negatively charged ones since the cells are generally negatively charged. | |||
In the brewing industry flocculation is a very important process in fermentation during the production of beer where cells form macroscopic flocs. These flocs cause the yeast to sediment or rise to the top of a fermentation at the end of the fermentation. Subsequently, the yeast can be collected (cropped) from the top (ale fermentation) or the bottom (lager fermentation) of the fermenter in order to be reused for the next fermentation. | In the brewing industry flocculation is a very important process in fermentation during the production of beer where cells form macroscopic flocs. These flocs cause the yeast to sediment or rise to the top of a fermentation at the end of the fermentation. Subsequently, the yeast can be collected (cropped) from the top (ale fermentation) or the bottom (lager fermentation) of the fermenter in order to be reused for the next fermentation. |