Difference between revisions of "Industrial fermentation"

* Phenylalanine

* Phenylalanine

The first step in the post-treatment of fermentation broth cultures, also known as '''downstream processing (DSP)''',  is to remove the cells from the medium.  This is typically performed by a solid-liquid separation technology, such, as [[centrifugation]] or [[membrane filtration]]. Each fraction can then undergo further processing, depending on whether the product is the biomass itself or an intra- or extracellular product. While intracellular products require cell disruption to release the products, extracellular products are solubilized in the depleted fermentation medium. Cell disruption techniques can be divided into mechanical methods (f.e. [[homogenisation]], [[Sizing|grinding]], [[Ultrasonication|sonication]], [[microwave treatment]], [[steam explosion]]) and non-mechanical methods (f.e. osmotic or temperature shock, [[Enzymatic processes|enzymatic destruction]]).  To further purify and concentrate the products several methods can be used including [[chromatography]], [[solvent extraction]], [[Crystallisation and precipitation|crystallization]], [[distillation]], [[drying]] etc. The choice of purification technology is depending on the characteristics of the desired products.

The first step in the post-treatment of fermentation broth cultures, also known as '''downstream processing (DSP)''',  is to remove the cells from the medium.  This is typically performed by a solid-liquid separation technology, such, as [[centrifugation]] or [[membrane filtration]]. Each fraction can then undergo further processing, depending on whether the product is the biomass itself or an intra- or extracellular product. While intracellular products require cell disruption to release the products, extracellular products are solubilized in the depleted fermentation medium. Cell disruption techniques can be divided into mechanical methods (f.e. [[homogenisation]], [[Sizing|grinding]], [[Ultrasonication|sonication]], [[microwave treatment]], [[steam explosion]]) and non-mechanical methods (f.e. osmotic or temperature shock, [[Enzymatic processes|enzymatic destruction]]).  To further purify and concentrate the products several methods can be used including [[chromatography]], [[solvent extraction]], [[Crystallisation and precipitation|crystallization]], [[distillation]], [[drying]] etc. The choice of purification technology is depending on the characteristics of the desired products.