Difference between revisions of "Solid state fermentation"

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==== Group 3: Rotating drum bioreactors ====
==== Group 3: Rotating drum bioreactors ====
Rotating drums bioreactors mix intermittently without forced aeration, operating on continuous or semi-continuous mode. A rotating drum bioreactor is a horizontal cylinder. The drum is semi-filled with a bed of substrate. The fermented bed cannot be too high and this creates good oxygen and carbon dioxide transfer. Temperature control also depends on the mixing effect on the solid substrate.
Rotating drum bioreactors mix intermittently without forced aeration, operating on continuous or semi-continuous mode. A rotating drum bioreactor is a horizontal cylinder. The drum is semi-filled with a bed of substrate. The fermented bed cannot be too high and this creates good oxygen and carbon dioxide transfer. Temperature control also depends on the mixing effect on the solid substrate.
[[File:Fluidized bedv2 SSF.png|thumb|Scheme of a gas-solid fluidized-bed bioreactor]]
[[File:Fluidized bedv2 SSF.png|thumb|Scheme of a gas-solid fluidized-bed bioreactor]]


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In order to maximize product yield, downstream strategies play an important role. The choice of downstream strategy is dependent of the type of product. For instance, if the product containts cellulase than the following downstream-based strategies can be used:<ref>{{Cite book|author=Darshan M. Rudakiya|year=2019|section_title=Strategies to Improve Solid-State Fermentation Technology|book_title=New and Future Developments in Microbial Biotechnology and Bioenegineering|publisher=Elsevier}}</ref>
In order to maximize product yield, downstream strategies play an important role. The choice of downstream strategy is dependent of the type of product. For instance, if the product containts cellulase than the following downstream-based strategies can be used:<ref>{{Cite book|author=Darshan M. Rudakiya|year=2019|section_title=Strategies to Improve Solid-State Fermentation Technology|book_title=New and Future Developments in Microbial Biotechnology and Bioenegineering|publisher=Elsevier}}</ref>


* Extraction (e.g., extraction buffer in case of extracellular cellulase production)
* [[Extraction]] (e.g., extraction buffer in case of extracellular cellulase production)
* Purification (e.g., protein precipitation methods, chromatography and filtration)
* Purification (e.g., protein precipitation methods, chromatography and filtration)
* Crystallization (i.e., inactivation and stabilization)
* Crystallization (i.e., inactivation and stabilization)