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Difference between revisions of "Field-Flow fractionation (FFF)"
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Field-Flow fractionation (FFF) (view source)
Revision as of 13:24, 2 September 2021
, 13:24, 2 September 2021→Centrifugal FFF
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==Process and technologies== | ==Process and technologies== | ||
Different variants of the FFF are available including tha Asymmetric flow FFF, centrifugal FFF, electrical FFF, split flow thin-cell fractionation (SPLITT), and thermal FFF. | Different variants of the FFF are available including tha Asymmetric flow FFF, centrifugal FFF, electrical FFF, split flow thin-cell fractionation (SPLITT), and thermal FFF. Depending on the applied technology particles can be separated in dependence of different physicochemical properties. | ||
===Asymmetric flow FFF (AF4)=== | ===Asymmetric flow FFF (AF4)=== | ||
[[File:AFFFF channel.svg|thumb|Illustration of a separation channel for asymmetric flow field-flow-fractionation.]] | [[File:AFFFF channel.svg|thumb|Illustration of a separation channel for asymmetric flow field-flow-fractionation.]] | ||
The asymmetric flow FFF (AF4) is realised in a separation channel where an separation force ist generated in form of an asymmetric crossflow through a semipermeable membrane. The injected particles can be eluted | The asymmetric flow FFF (AF4) is realised in a separation channel where an separation force ist generated in form of an asymmetric crossflow through a semipermeable membrane. Through the cross-flow larger particles accumulate at the channel bottom while smaller particles accumulate at the center of the channel. The injected particles can be eluted through a parabolic flow-profile in combination with the reduction of the crossflow. | ||
=== Centrifugal FFF === | === Centrifugal FFF === | ||
In centrifugal FFF the separation force is realised via an centrifugal field. Through the induced gravitational field larger particles accumulate at the channel bottom while smaller particles accumulate at the center of the channel. The injected particles can be eluted through a parabolic flow-profile in combination with the reduction of the centrigigal field. | |||
=== Electrical FFF === | === Electrical FFF === | ||