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Difference between revisions of "Field-Flow fractionation (FFF)"
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Field-Flow fractionation (FFF) (view source)
Revision as of 13:46, 2 September 2021
, 13:46, 2 September 2021→Split flow thin-cell fractionation (SPLITT)
Lars Krause (talk | contribs) |
Lars Krause (talk | contribs) |
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==Feedstock== | ==Feedstock== | ||
Suitable feedstocks are heterogeneous mixtures of different substances in form of dilute suspensions (solids in liquid). Depending on the applied process and technology solids can be usually separated between the nm-µm range. The FFF is usually applied to separate nanoparticles, polymers, and proteins for analytical and preparative purposes. | Suitable feedstocks are heterogeneous mixtures of different substances in form of dilute suspensions (solids in liquid). Depending on the applied process and technology solids can be usually separated between the nm-µm range. The FFF is usually applied to separate cells, different kind of nanoparticles, polymers, and proteins for analytical and preparative purposes. | ||
==Process and technologies== | ==Process and technologies== | ||
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=== Centrifugal FFF === | === Centrifugal FFF === | ||
In centrifugal FFF the separation force is realised via an centrifugal field. Through the induced gravitational field larger particles accumulate at the channel bottom while smaller particles accumulate at the center of the channel. The injected particles can be eluted through a parabolic flow-profile in combination with the reduction of the | In centrifugal FFF the separation force is realised via an centrifugal field. Through the induced gravitational field larger particles accumulate at the channel bottom while smaller particles accumulate at the center of the channel. The injected particles can be eluted through a parabolic flow-profile in combination with the reduction of the centrifugal field. Due to the large range of applicable centrifugal force the method has its advantage to separate a wide range of different sized particles (usually µm-nm range). | ||
=== Electrical FFF === | === Electrical FFF === | ||
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=== Split flow thin-cell fractionation (SPLITT) === | === Split flow thin-cell fractionation (SPLITT) === | ||
In Split flow thin-cell fractionation (SPLITT) earth's gravitational force is used to separate different sized particles (usually in µm-range). Usually the suspensions are introduced into the top of a separation channel while a carrier liquid is pumped into the channel from the bottom. The separation of different sized solids occurs along the channel induced by earth's gravity. Two outlets (one at the channel bottom, one at the channel top) at the end of the channel separates the particles into a larger and smaller fraction while the cut-off can be controllel via the channel flows. | |||
=== Thermal FFF === | === Thermal FFF === | ||
Possibly not relevant | |||
==Product== | ==Product== | ||